Journal of Clinical Oncology, 2004 ASCO Annual Meeting Proceedings (Post-Meeting Edition).
Vol 22, No 14S (July 15 Supplement), 2004: 9710
© 2004 American Society of Clinical Oncology

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Abstract

Evaluation of pro-angiogenic and pro-coagulant activity of irradiated and non irradiated stored blood used in transfusion practice

Blood Bank and Insts.of Thrombosis and Oncology, Sheba Medical Center,Tel Hashomer, Israel; Sheba Medical Center, Tel Hashomer, Israel

9710

Background: Side effects of blood transfusion may be associated with infusion of bioactive substances. It has been previously reported that increase in pro-angiogenic and pro-coagulant parameters was observed in plasma of patients transfused with blood and platelet concentrates, respectively. Irradiation of cellular blood components is currently the only acceptable method for prevention of transfusion-associated GVHD. The aim of this study was to evaluate the pro-angiogenic and pro-coagulant potential of stored non-irradiated and irradiated blood. Methods: Ten units of whole blood, 4 of them irradiated at day 7 of storage (2500 rad), were studied. VEGF, thrombin antithrombin complex (TAT), prothrombin fragments 1+2 (F1+2) and endostatin were measured. K and LDH served as parameters of hemolysis. Results: An increase in VEGF was observed with increase in storage time (p by ANOVA = 0.016 and 0.018 for non irradiated and irradiated units, respectively). An increase was more pronounced in irradiated units (p<0.001). No difference in the endostatin level was observed throughout the study (p = 0.259 and 0.326 by ANOVA for non irradiated and irradiated units, respectively). A statistically significant increase in F1+2 and TAT was observed only in stored irradiated units (p= 0.013 for F1+2 and 0.0109 for TAT by ANOVA) with significant difference between the non-irradiated and irradiated units (p = 0.001 for each F1=2 and TAT). Similarly, a significant increase in thrombin generation in irradiated units was observed using a different method (Thromb Haemost 2000; 83:589). An increase in hemolytic parameters was observed in all the units regardless of irradiation (p=0.007 and 0.002 for K in non-irradiated and irradiated units, respectively; p=0.003 and <0.001 for LDH in non-irradiated and irradiated units, respectively). Conclusions: 1. Storage of blood is associated with an increase in pro-angiogenic factor -VEGF with further rise in its levels by irradiation. 2. Storage of irradiated blood is associated with an increase in thrombin generation resulting in potential pro-coagulant activities of the transfused units.

No significant financial relationships to disclose.